Nucleate works with emerging founders who leverage life sciences to transform industries

Key figures

Key figures

Number of past
participating companies

Million USD
funds raised to date

Number of
jobs created

“Nucleate was an incredible opportunity to get hands-on experience learning the components of building a biotech as well as unparalleled access to mentors and resources. It’s an absolute must if you’re interested in biotech entrepreneurship.”
CAROLINE DAUGHERTY
HBS • SEORX
“I joined Nucleate to get exposure to early stage life company formation. The program connected me with my now Wayne, and offered us a risk-free way to validate our It gave us an opportunity to develop our story, practice it and receive invaluable feedback from the most investors in the space.”
HUNTER GOBLE
HBS • KARIVEZ
“Nucleate was an incredibly educational and eye-opening experience. Each session was led by biotech superstars and I learned so much from my team of MBAs/PhDs. I gained hands-on experience in many different aspects of the start-up process from pitching to VCs to building an operational budget. I would highly recommend the experience to anyone looking to jump into a career in biotech!”
JULIA PIAN
HBS • MANIFOLD BIO
“Nucleate Bio is rigorous and tough, but the materials the work produces are invaluable. Anyone serious about a startup should try hard to get into this program.”
JONATHAN RITTICHIER
WYSS INSTITUTE • ENPLUS ONE BIOSCIENCES
“The Nucleate program has been an incredible tool to refine our business model, building a solid network, and crafting a convincing pitch deck. I would recommend it to any scientist willing to explore the potential impact of her/his work in the biotech market.”
DANIELE FORESTI & EVERETT FROST
HMS & WYSS - HBS • ACOUSTICA BIO
“The Nucleate Program was instrumental in connecting our team to mentors who genuinely cared about our progress. Additionally, the regular events with industry experts and venture capitalists helped us ensure that we were framing our problem, solution, market, and milestones in a context that would be appealing to future funding sources. By the end of the program, we had constructed a strong pitch deck that led to several additional opportunities. On a personal note, I greatly enjoyed connecting with the other researchers and MBA students, many of whom I may never have met if not for the Nucleate Program.”
DANIELE FORESTI & EVERETT FROST
HMS & WYSS - HBS • ACOUSTICA BIO
“Nucleate was key to providing a scaffold for the team to get out of the lab and put together a viable business model. Some of the highlights of the program included mentorship from experienced biotech operators and company builders, feedback prioritizing application areas from the mapping experts at RA Capital, and finally putting it all together to pitch a mix of investors from both traditional biotech models and newer accelerators.”
GLEB KUZNETZOV & PIERCE OGDEN
HMS & WYSS • MANIFOLD BIO
“Nucleate helped launch our research idea into an exciting commercial opportunity! The value of Nucleate lies in its great leadership, extensive life sciences connections, and structured programming which was critical for the development of our business strategy and pitch to prospective investors.”
NATALIE FERGUSON & IVANA SHUI
HMS & HBS • SALVOS
“Nucleate is the reason Asan Therapeutics exists – this program is not just how we met but what enabled us to develop and chase dreams of entrepreneurship. We have had access to so many incredible mentors and opportunities that continue to help us shape our venture.”
ANANYA ZUTSHI & MORTEZA SARMADI
HBS & MIT • ASAN THERAPEUTICS

Current cohort

3DDiagnostix San diego
Launched 2022
Diagnostics
3D DiagnostiX aims to produces and develop transformative diagnostic tools for protein structure characterization and for early detection of Alzheimer’s and other misfolding diseases in which the protein structure is altered.
3DOE Boston
Launched 2022
Therapeutics
3D bioprinting platform using matrix compaction and sacrificial ink to create perfusable, high-density engineered tissue; initially targeting vascularized fat tissue free flaps for reconstructive surgery
Abacus Bio Baltimore
Launched 2022
Platform
Abacus Bio is building a deep learning platform for rapid development of next-generation antibody therapeutics.
Acurasset Therapeutics Boston
Launched 2022
Therapeutics
Small-molecule therapeutic for hepatitis B with the differentiated approach of inhibiting cccDNA formation and transcription to terminate the virus life cycle within liver cells.
Aephoris Boston
Launched 2022
Eco
Tolerance-enhanced oleaginous yeast for high volume cellulosic diesel.
Altaios All locations
Launched 2022
Platform
Altaios is early-stage disruptor with the mission to bring simple and more accurate women's health diagnostic tools integrated with telehealth.
Altuna Bayarea
Launched 2022
Platform
Leveraging DNA sequencing to perform multiplexed protein quantification, quantifying dozens of proteins in over 10,000 patient samples in a single run, and reducing the cost of protein detection 10-100 fold
Aperture Biosciences Boston
Launched 2022
Medical Devices
Near-real-time molecular analysis platform using repeated staining with antibody-associated fluorochromes; targeting quantification of the types and abundance of cancer and immune cells in tumor microenvironments
Ascent Bio New haven
Launched 2022
Platform
A robust AI-based platform for the generation and highly controlled optimization of diverse classes of therapeutics
Biosens8 Boston
Launched 2022
Medical Devices
Biosensor engineering platform using bacterial allosteric transcription factor - quantum-dot FRET and electrochemical transduction techniques; targeting ovulation confirmation in fertility medicine
Biospoke New york city
Launched 2022
Platform
Chromosome synthesis at unprecedented scale for precision gene circuit engineering.
BioSynthetica San diego
Launched 2022
Platform
Our technology consists of a bacterial genetic circuit for the inducible synchronized delivery of any molecule of interest for applications in the microbiome space.
Biped Therapeutics New york city
Launched 2022
Therapeutics
Next-generation proteolysis targeting chimeras.
Bonsai BioForge Boston
Launched 2022
Eco
Engineered microbes for efficient biomanufacturing
CacheDNA Boston
Launched 2022
Platform
Building the next generation of DNA-based storage and computing: cheaper, more robust, more energy efficient, long-term storage and random access of DNA, making the world’s molecular data more widely accessible and useful.
Calliope Biofoundry New haven
Launched 2022
Platform
A platform for redesigning microbes using synthetic biology, and harnessing their unique capabilities for applications in therapeutics, materials, energy, food, and agriculture
Cerebrus Biosciences Bayarea
Launched 2022
Platform
Overcoming gene therapy hurdles with a novel class of gene editors
Cogito Biosystems Los angeles
Launched 2022
Medical Devices
Focused ultrasound delivery of RNA therapeutics
Coral Therapeutics Boston
Launched 2022
Platform
Therapeutic target discovery platform combining lung-organoids with graph-based AI algorithms to predict and experimentally validate target candidates; initially working in lung cancer.
Correlimmune New york city
Launched 2022
Diagnostics
Novel strategy for dried blood spot testing, targeting COVID and other urgent diseases.
curIT Biotech Los angeles
Launched 2022
Therapeutics
Anti-CD64 human cytolytic fusion proteins (hCFPs) for the treatment of acute myeloid leukemia
DNAli Data Research triangle
Launched 2022
Platform
DNAli Data has developed new technologies to write and access data in DNA molecules. This project will create a next-generation data storage technology using DNA as the information substrate.
Dominant Therapies Boston
Launched 2022
Therapeutics
Antiviral therapeutic using a preferentially expressed, or dominant, mRNA encoding a fusion-modified protein that degrades the viral genome to combat viral evolution; initially targeted to SARS-CoV2 (COVID-19)
DoriVac Boston
Launched 2022
Platform
Vaccine development platform leveraging DNA origami nanotechnology to stimulate stronger immune response; targetable to both malignancy (e.g., cancer) and infectious disease
Encreto Therapeutics Boston
Launched 2022
Platform
Organoid-based endocrine drug discovery platform using human GI stem cells coupled with high-throughput screening for inducers of enteroendocrine cell function, initially targeting obesity and type 2 diabetes
FluoroLife Boston
Launched 2022
Medical Devices
Optical imaging device leveraging differential fluorescence-lifetime to better distinguish tumor margins from healthy tissue; initially targeting solid tumor resection
Gammunity Los angeles
Launched 2022
Therapeutics
Memory CAR-Gamma delta T cells against solid tumors
Graph Bio Bayarea
Launched 2022
Platform
Using AI applications to identify suitable patients for clinical trials based on health records
Healcisio San diego
Launched 2022
Medical Devices
Healcisio seeks to improve patient care through the early prediction of sepsis using clinically actionable deep learning.
Hybridex Biosciences New haven
Launched 2022
Therapeutics
Synthetic nucleic acid technology capable of fine-tuning CRISPR/Cas9 cutting and reducing off-target editing.
HydroGene Baltimore
Launched 2022
Medical Devices
HydroGene developed a non-viral gene therapy system leveraging hydrodynamic delivery of DNA through endoscopic procedures.
Hypermelt Baltimore
Launched 2022
Diagnostics
HyperMelt developed a sensitive microfluidics platform for detection of extremely rare DNA from non-invasively collected samples, such as tumor-derived DNA for early cancer detection or sepsis-inducing bacteria in blood.
Immunobac Therapeutics Boston
Launched 2022
Platform
Antibacterial target identification platform leveraging transposon library screening and machine-learning methods to inform complement-directed host-informed therapies (i.e. targeted antibodies)
InGel Therapeutics Boston
Launched 2022
Therapeutics
Bioinspired hydrogel platform engineered for the sustained release of therapeutics in the eye, with tunable stiffness and low immunogenicity; initially targeting macular degeneration and geographic atrophy
INIA Biosciences Boston
Launched 2022
Medical Devices
Ultrasound device for modulating inflammation paired with biomarker-based monitoring system feeding an AI-driven treatment optimization algorithm. Initially targeting organ rejection and chronic inflammatory disease
Innervate Boston
Launched 2022
Therapeutics
IL-3 therapeutic for microglia survivability and neuronal fiber regeneration; initially targeting neurotrophic keratitis with potential application to broader neuropathic pain and neurodegeneration
IrNex Research triangle
Launched 2022
Medical Devices
Irnex-Inc has developed phase-incrementing MR Spectroscopic Imaging (pi-MRSI) technology for fast biomarker imaging to improve cancer detection specificity and monitor treatment responses.
Junevity Bayarea
Launched 2022
Therapeutics
Rejuvenating old skin cells through gene therapy by targeting novel genes without causing cancer
Kalia Health Research triangle
Launched 2022
Medical Devices
Kalia Health designed the Kal-PDx strip, a non-invasive, rapid, at-home diagnostic kit for early detection of preeclampsia (PE). Early PE detection allows for a safer pregnancy
Kova Dx Bayarea
Launched 2022
Diagnostics
Computer-vision based point-of-care diagnostic and monitoring for sickle cell disease and other blood disorders
Kovo Biosciences San diego
Launched 2022
Platform
Kovo Bioscience is pioneering the development of highly specific antiviral therapies to prevent and treat viral infections using our novel RNA-targeting platform.
Lipid X Bayarea
Launched 2022
Platform
A novel vaccine platform for increased immunity to highly mutagenic viruses
Lucem Bayarea
Launched 2022
Platform
Revolutionizing tissue, cell, and receptor targeting with photoswitch based drug delivery
Lymphagen Los angeles
Launched 2022
Therapeutics
Retinoic acid to prevent post-surgical lymphadema
MacPatch Boston
Launched 2022
Therapeutics
Macrophage modulation platform leveraging cell-adhesive polymer matrix to deliver IFN-y for polarizing macrophages into target phenotypes; initially targeting hepatic fibrosis
Micro-Niche Research triangle
Launched 2022
Platform
Micro-Niche developed a biochemical technology called IDE (Inducible Directed Evolution) that creates useful mutations in E. coli bacteria, including improving the yield of enzymes and colonization in the mammalian gut.
MIDAS Boston
Launched 2022
Eco
Material recovery from municipal solid waste incinerated ash
MyoArete All locations
Launched 2022
Therapeutics
MyoArete is a startup focused on developing a cure for Duchenne Muscular Dystrophy (DMD) targeting utrophin using small molecule medicines
NAD Oncology New york city
Launched 2022
Therapeutics
Small molecule therapeutic company targeting breakthrough understandings of oncometabolism against solid tumors.
Nascent Genomics San diego
Launched 2022
Diagnostics
Nascent Genomics is leveraging sequencing of the entire nascent transcriptome to develop next generation diagnostics and identify novel biomarkers for disease.
Neurocure Research triangle
Launched 2022
Platform
Neurocure developed DANNCE, an AI platform for quantifying behavior and detecting neurological movement disorders in humans and animals using scalable 3D movement analysis. Our high throughput and data driven approach will allow for more objective diagnosis of disease, earlier detection of disease, evaluating the efficacy of pharmacological agents, and the ability to quantify a subject’s response to treatment.
Nitro Bio All locations
Launched 2022
Platform
Nitro Bio is a platform technology startup that leverages live vaccine response enhancement through selectively modifying residues on surface expressed antigens with immunogenic groups
Optimer Biotech New york city
Launched 2022
Platform
Discovery engine combining advanced biophysical modeling and high-throughput screening.
Orafay Los angeles
Launched 2022
Medical Devices
Microneedle therapy for the treatment of reverse gum deterioration
OrganOmics Bayarea
Launched 2022
Platform
We have engineered 3D human tissues that better mimic tissues of the human body for drug discovery
Osteologic New york city
Launched 2022
Platform
Drug discovery leveraging bone-targeted therapeutics for novel systemic delivery.
Paracyte Bio Boston
Launched 2022
Therapeutics
Parasite-drived immunomodulatory therapeutic platform selecting candidates from AI-predicted libraries with high-throughput screens; initially targeting complement inhibitors and cytokine binding proteins
Perform Ab Baltimore
Launched 2022
Platform
PerformAb developed a technology to screen antibodies on the surface of yeast cells against mammalian cells expressing the target membrane proteins and applied for targeted therapeutics.
PlanTure San diego
Launched 2022
Eco
Crop genomics to reduce plant breeding times
Plantwriter Bayarea
Launched 2022
Eco
Machine learning and CRISPR/Cas9 to exponentiate the pace of crop improvement
Project Sparkle Boston
Launched 2022
Platform
Revolutionary new binder platform technology leveraging non-standard amino acids. Initial application development includes faster research tools, improvements upon existing technologies, and novel diagnostics.
Prompt Diagnostics Baltimore
Launched 2022
Diagnostics
Prompt Diagnostics is developing a portable sample-to-answer platform for rapid, low-cost detection of disease biomarkers and infectious pathogens.
PulseFlex Boston
Launched 2022
Medical Devices
Wearable, low-power blood pressure monitor using a piezoelectric nanofiber array for pressure sensing and a machine learning algorithm for analysis of the spatial-temporal waveforms of a near-surface artery
ReBreathe Los angeles
Launched 2022
Platform
G-protein coupled receptor stimulation for opioid addiction treatment
Reel Seafoods Boston
Launched 2022
Eco
Cell-based seafood with 3D bioprinting to improve texture and scale
Resistorium Bayarea
Launched 2022
Eco
Novel plant immune receptors that will protect crops from pests and diseases
Rugged Bio Boston
Launched 2022
Eco
Non-model microbes to improve bioproduction
Sapien Biosciences New york city
Launched 2022
Platform
Better models of human disease and screening platform using prime editing.
Sonocure Research triangle
Launched 2022
Therapeutics
Sonocure developed a transformative topical and non-invasive therapy to eradicate wound infection using novel antibiotic adjuvants and ultrasound-stimulated phase-change agent.
StoneFish Bio Bayarea
Launched 2022
Platform
Uses a novel subset of natural killer cells to generate a cell product with high levels of cytotoxicity, tumor infiltration, and cytokine secretion to address current limitations of solid tumor immunotherapies
Sun Serum San diego
Launched 2022
Eco
Sustainable alternatives for growth factors leveraging the diversity within the plant kingdom
Switch Bio Los angeles
Launched 2022
Platform
Conditional Guide RNA for Gene Editing and Drug Discovery.
Talos Tx San diego
Launched 2022
Therapeutics
Talos Tx is building small molecule therapeutics targeting key, undrugged mechanisms for patients with neurodegenerative diseases.
Tenacity Boston
Launched 2022
Platform
Genetic circuit platform resistant to epigenetic silencing, enabling synthetic biology within cell therapies to function over longer time scales than traditional methods. Initially targeting CAR-T exhaustion
uPROCESSOR Therapeutics Boston
Launched 2022
Therapeutics
Genetic switch platform for conditional expression of therapeutic microRNA tied to biomarker presence or absence; initially targeting solutions to CAR-T inactivation and cytokine release syndrome
UTR Therapeutics New york city
Launched 2022
Therapeutics
Precise control of post-transcriptional regulation against hard-to-treat cancers.
Vitra Labs Bayarea
Launched 2022
Platform
Leveraging novel stem cell induction protocol to revolutionize in vitro fertilization
Wake Therapeutics Bayarea
Launched 2022
Platform
Generating new therapeutic strategies by identifying and targeting RNA regulatory elements
Woodpack Boston
Launched 2022
Eco
New naturally compostable plastic film with multi-use application
3DDiagnostix
3DOE
Abacus Bio
Acurasset Therapeutics
Aephoris
Altaios
Altuna
Aperture Biosciences
Ascent Bio
Biosens8
Biospoke
BioSynthetica
Biped Therapeutics
Bonsai BioForge
CacheDNA
Calliope Biofoundry
Cerebrus Biosciences
Cogito Biosystems
Coral Therapeutics
Correlimmune
curIT Biotech
DNAli Data
Dominant Therapies
DoriVac
Encreto Therapeutics
FluoroLife
Gammunity
Graph Bio
Healcisio
Hybridex Biosciences
HydroGene
Hypermelt
Immunobac Therapeutics
InGel Therapeutics
INIA Biosciences
Innervate
IrNex
Junevity
Kalia Health
Kova Dx
Kovo Biosciences
Lipid X
Lucem
Lymphagen
MacPatch
Micro-Niche
MIDAS
MyoArete
NAD Oncology
Nascent Genomics
Neurocure
Nitro Bio
Optimer Biotech
Orafay
OrganOmics
Osteologic
Paracyte Bio
Perform Ab
PlanTure
Plantwriter
Project Sparkle
Prompt Diagnostics
PulseFlex
ReBreathe
Reel Seafoods
Resistorium
Rugged Bio
Sapien Biosciences
Sonocure
StoneFish Bio
Sun Serum
Switch Bio
Talos Tx
Tenacity
uPROCESSOR Therapeutics
UTR Therapeutics
Vitra Labs
Wake Therapeutics
Woodpack

Past cohorts

Glyphic Bio Boston
Launched 2021
Platform
Protein sequencing platform for detection and identification of low-expression proteins, potentially including novel therapeutic targets.
AptaBridge Boston
Launched 2021
Therapeutics
Aptamer design platform enabling linkage of monospecific components to create multi-specifics or alter pharmacokinetics, targeting immunoncology.
Kano Therapeutics Boston
Launched 2021
Platform
DNA engineering platform leveraging M13 bacteriophage for scalable construction of gene-length ssDNA, targeting therapeutic production.
Concerto Biosciences Boston
Launched 2019
Therapeutics
Patented, data-driven platform for engineering microbial communities with useful properties.
NextRNA Boston
Launched 2019
Platform
NextRNA is transforming the non-coding RNA therapeutic space and is shifting the concept of undruggable into druggable.
Manifold Bio Boston
Launched 2019
Platform
A novel experimental platform that generates pharmacological measurements on millions of therapeutic protein variants in parallel.
Acoustica Bio Boston
Launched 2019
Platform
Improving clinical care of millions of patients by reformulating existing intravenously-delivered drugs in a subcutaneous form, using a patented acoustophoretic printing approach.
Beacon Biomimetics Boston
Launched 2020
Medical Devices
Developd PhonoGraft, a novel device composed of a synthetic, biodegradable elastomer that can restore biomimetic stiffness in the tympanic membrane to improve hearing outcomes.
PionEar Technologies Boston
Launched 2020
Medical Devices
Developing a new generation of fluid-guiding and biocontamination-resistant tympanostomy tubes that allow antibiotic drops to penetrate the middle ear more efficiently.
Limax Bio Boston
Launched 2021
Diagnostics
Tissue adhesive combining high strength, high adhesion energy, and biocompatibility for wound healing across multiple tissue types.
Ilesa Bio Boston
Launched 2021
Therapeutics
Biotherapeutic for shielding native gut microbiota from antibiotics, for prevention of dysbiosis-related inflammatory and metabolic disorders.
Crisscross Nano Boston
Launched 2021
Diagnostics
Diagnostic platform leveraging DNA nanostructures for rapid, low-cost detection of nucleic acids and proteins, initially targeting infectious disease.
Carmel Medicine Boston
Launched 2021
Therapeutics
Therapeutic neural growth factor (NGF) addressing the historically dose-limiting side effect of pain, initially targeting peripheral neuropathy.
PhageLink Boston
Launched 2021
Medical Devices
Genetically engineered, biocompatible hydrogel that is customizable for broad therapeutic applications in drug delivery and 3D printing in vivo.
Kumuda Boston
Launched 2021
Platform
In silico drug discovery platform analyzing protein interaction, structure, and drug target homology across the human genome to discover drug targets.
MicroLife Boston
Launched 2021
Eco
Creating a stable probiotic spray using a patented silk and trehalose technology to deliver an engineered microbe microenvironment.
Syntrophia Boston
Launched 2021
Eco
Creating assay kits to continuously monitor and supercharge biomanufacturing and fermentation processes using FISH-TAMB mRNA imaging technology.
Glyphic Bio
AptaBridge
Kano Therapeutics
Concerto Biosciences
NextRNA
Manifold Bio
Acoustica Bio
Beacon Biomimetics
PionEar Technologies
Limax Bio
Ilesa Bio
Crisscross Nano
Carmel Medicine
PhageLink
Kumuda
MicroLife
Syntrophia